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BRCF Proteomics Resource Facility (PRF)
dedicated to protein analysis and applying mass spectrometry-based proteomic approaches to varied biological questions.
Contacts
Venkatesha Basrur, PhD
734-615-5722
[email protected]
Location
MSRB II (Rm 3554/3556)
1150 Catherine St, Ann Arbor, MI
Medical School
Who We Serve
University of Michigan Researchers
Core Summary
PRF is directed by Dr. Alexey Nesvizhskii, who is a world leading expert in the area of mass spectrometry bioinformatics and large-scale proteomics. Technical expertise in mass spectrometry is provided by dedicated a full-time research faculty, namely, Dr. Venkatesha Basrur and a senior technical assistant, Mr. Kevin Conlon, both of whom have extensive experience in biological mass spectrometry.
Services
- Data-Independent Acquisition (DIA)
- Identification of protein bands from Coomassie and mass spectrometry-compatible silver-stained gels
- Mass spectrometry
- Parallel Reaction Monitoring (PRM) for quantification of proteins and post-translational modifications
- Post-translational modifications mapping
- Protein quantitation
Label-free quantitation, Parallel reaction monitoring, SILAC quantitation, TMT quantitation,
- Proteomics
- Quantitative proteomic profiling
- Shot-gun proteomic analysis of complex mixtures by tandem mass spectrometry
Equipment
- Orbitrap Fusion Tribrid with ETD
- Q Exactive HF