Michigan Diabetes Research Center (MDRC) Microscopy, Imaging, and Cellular Physiology Core (MICP)

provides protein/RNA imaging and cell physiological analysis for diabetes-related research.

Contacts

David A. Antonetti, PhD
734-232-8230
dantonet@med.umich.edu

Stephen Lentz, PhD
734-647-8233
lentzs@umich.edu

Location

Brehm Tower (Rm 6245)
1000 Wall St, Ann Arbor, MI

Med Sci II (Rm 7732B)
1137 Catherine St, Ann arbor, MI

Affiliations
Medical School

Who We Serve

University of Michigan Researchers

Core Summary

Microscopy, Imaging, and Cellular Physiology Core (MICP Core) of the Michigan Diabetes Research Center (MDRC). The MICP Core provides state-of-the-art protein and RNA imaging and cell physiological analysis to enhance the diabetes-related research at the University of Michigan. The MICP Core provides expertise, advice, training and equipment for microscopic and morphometric analysis of both protein and RNA in biologic samples in support of research focused on diabetes and its complications or related endocrine and metabolic disorders. The MICP Core provides access to a wide variety of microscopy, imaging and electrophysiology equipment that otherwise would be beyond the scope and resources of most investigators. The overwhelming majority of MDR Core investigators who require such techniques rely on the MICP Core to provide them and their staff with expert consultation, training and support for routine and advanced microscopy and image analysis for protein and RNA morphometric studies and for optogenetic and electrophysiology studies.

Use of the Optogenetic and Electrophysiology laboratory begins with consultation with Dr. Goforth for discussion of goals and feasibility. Dr. Goforth will then work with the MDRC investigator to devise an experimental plan and generate electrophysiology data. Extended studies will require training of laboratory personal by Dr. Goforth in the laboratory of the MDRC investigator.

Service Categories: Histology, Pathology and Tissue Service Categories: Imaging

Services

Cellular Physiology Laboratory
Consultation and training for data analysis and interpretation, Consultation to develop experimental design, Ex vivo live-cell calcium imaging in conjunction with the Imaging Laboratory, optogenetic and/or electrophysiologic experiments for the generation of pilot data and/or small project collaboration,

Imaging Laboratory
Access to Widefield and Confocal Microscopes for Fixed and Live Cells and Tissues, Access to Windows PC Workstations for Image Analysis, Consultation and Training for Image Analysis, Consultation and Training for Widefield and Confocal Microscopy,

In Situ Hybridization Laboratory
Complete ISH procedure (hybridization and signal detection), Consultation, Data analysis, Dual label ISH / immunohistochemistry, Probe labeling by in vitro transcription, Tissue processing,


Equipment

Cellular Physiology Laboratory
a multiClamp 700B patch-clamp amplifier and Axon Digidata 1550 Data Acquisition system, a temperature control and perfusion system, including; two Sutter instruments MP-285 micromanipulators mounted on a Burleigh Gibraltar stage,

Imaging Laboratory
3D Imaris, 3D Volocity, AutoQuant deconvolution, ImageJ, Leica SP5 Inverted Confocal Microscope, MATLAB, MetaMorph, Nikon Inverted TiE Widefield and A1 Confocal Microscope, Offline Leica Application Suite (LAS X), Offline Nikon Elements, Olympus Fluoview 500 Inverted Confocal Microscope, Windows PC Workstations for Image Analysis,

In Situ Hybridization Laboratory
Dual Label In Situ Hybridization and Immunohistochemistry, Fluorescence: RNAprobe using RNAscope Technology, Radioisotopes: Autoradiographic Film and Emulsion,