BRCF Metabolomics Core

measures concentrations of small molecules in biological samples on a recharge basis.

Contacts

Maureen Kachman, PhD
734-232-0842
mkachman@umich.edu

Location

6120 Brehm Tower
1000 Wall St, Ann Arbor, MI

Affiliations
Medical School

Who We Serve

University of Michigan Researchers

Core Summary

The BRCF Metabolomics Core measures concentrations of small molecules in biological samples on a recharge basis. We strive to provide quality analytical services at competitive prices, and regularly customize assays to fit our clients’ needs. Services include initial consultation, method development, data collection, interpretation, and presentation. Core competencies include both targeted assays for many analyte classes as well as untargeted assays to evaluate the entire metabolome, the measurement of flux through various pathways using 13C-isotopomer analyses, and structural identification of isolated unknowns and potential biomarkers

Services

  • Bioinformatics analysis

  • Method Development

  • Non-targeted Assays
    Central Carbon Metabolism, One-carbon Metabolism, Reversed Phase (Positive and Negative),

  • Shotgun Lipidomics (Non-targeted Lipidomics)

  • Stable Isotope Labelling (Fluxomics) of any existing targeted assay

  • Statistics

  • Targeted Assays
    Acylcarnitines, Amino Acids, Bile Acids, D4-Steroids, Eicosanoids, Glycolysis/TCA, Short Chain Fatty Acids,


Equipment

  • GC-MS 6890
    Agilent, Core Use Only
  • LC-QQQ 6410
    Agilent, Core Use Only
  • LC-QQQ 6490
    Agilent, Core Use Only
  • LC-QTOF 6545
    Agilent, Core Use Only
  • LC-TripleTOF 5600+
    Sciex, Core Use Only
  • Orbitrap IDX
    Thermo, Core Use Only