Human Stem Cell and Gene Editing Core (HSCGE)

provides iPSC reprogramming, stem cell characterization and differentiation, and genome editing.

Contacts

Wei Niu, PhD
734-615-8215
weiniu@med.umich.edu

Location

BSRB (Rm 5371)
109 Zina Pitcher Pl, Ann Arbor, MI

Affiliations
Medical School

Who We Serve

University of Michigan Researchers and External Researchers

Core Summary

Founded in 2015, the HSCGE core is a well-established research core facility at University of Michigan Medical School. Our state-of-the-art facility is located on the 5th floor of BSRB. We provide a wide range of high-quality services (iPSC reprogramming, stem cell characterization, genome editing, and stem cell differentiation) and access to advanced instrumentation, methodologies and expertise. Our personnel are very friendly and extremely well trained in stem cell biology as well as neuroscience applications. We serve investigators from UM, and researchers from external research centers as well as industry throughout the United States. We work with both the IRB and the HPSCRO committees and can provide regulatory advice to investigators.

Service Categories: Cell-Based Research Service Categories: Molecular Biology

Services

2D monolayer differentiation
cortical excitatory neurons, dopaminergic neurons, inhibitory neurons, motor neurons,

3D organoid differentiation
cortical organoid, thalamus organoid, ventral organoid,

CRISPR/Cas9 genome/gene editing
knock in point mutations and epitope tagging (HDR), knockout (NHEJ),

Facility usage

Generate iNeurons
cortical excitatory neurons, inhibitory neurons,

hESC/hiPSC expansion/freezing

iPSC derivation from patients and controls
skin fibroblast, whole blood,

iPSC lines
female, male,

Neuronal network activity assays on neurons derived from human stem cells

Training
hESC/hiPSC maintenance, hESC/hiPSC transfection,


Equipment

Biosafety cabinet

Clean bench

Live-Cell Analysis
EVOS cell imaging system, IncuCyte living-cell analysis system,

Multiple electrode array (MEA)-Axion BioSystems

Tissue culture incubator
and hyperoxia, hypoxia, regular,

Transfection
Invitrogen NEON transfection system,