Human Stem Cell and Gene Editing Core (HSCGE)

provides iPSC reprogramming, stem cell characterization and differentiation, and genome editing.

Contacts

Wei Niu, PhD
734-615-8215
weiniu@med.umich.edu

Location

BSRB (Rm 5371)
109 Zina Pitcher Pl, Ann Arbor, MI

Affiliations
Medical School

Who We Serve

University of Michigan Researchers and External Researchers

Core Summary

Founded in 2015, the HSCGE core is a well-established research core facility at University of Michigan Medical School. Our state-of-the-art facility is located on the 5th floor of BSRB. We provide a wide range of high-quality services (iPSC reprogramming, stem cell characterization, genome editing, brain organoids, and stem cell differentiation) and access to advanced instrumentation, methodologies, and expertise. Our personnel are very friendly and extremely well trained in stem cell biology as well as neuroscience applications.  We serve investigators from UM, and researchers from external research centers as well as industry throughout the United States. We work with both the IRB and the HPSCRO committees and can provide regulatory advice to investigators.

Service Categories: Cell-Based Research; Molecular Biology

Services

  • 2D monolayer differentiation
    cortical excitatory neurons, dopaminergic neurons, inhibitory neurons, motor neurons,

  • 3D organoid differentiation
    cortical organoid, thalamus organoid, ventral organoid,

  • CRISPR/Cas9 genome/gene editing
    knock in point mutations and epitope tagging (HDR), knockout (NHEJ),

  • Facility usage

  • Generate iNeurons
    cortical excitatory neurons, inhibitory neurons,

  • hESC/hiPSC expansion/freezing

  • iPSC derivation from patients and controls
    skin fibroblast, whole blood,

  • iPSC lines
    female, male,

  • Neuronal network activity assays on neurons derived from human stem cells

  • Training
    hESC/hiPSC maintenance, hESC/hiPSC transfection,


Equipment

  • Biosafety cabinet
    Thermo, Core Use Only
  • Centrifuge Sorval ST8
    Thermo, Equipment Available for Use
  • Countess FL
    Thermo, Core Use Only
  • ECO CLEANBENCH
    Thermo, Core Use Only
  • EVOS FL Auto live imaging system
    Thermofisher, Equipment Available For Use
  • Heraeus CO2 tissue culture incubators
    Thermo, Core Use Only
  • IncuCyte living-cell analysis system
    Sartorius, Equipment Available For Use
  • Inverted CKX53 microscope with LED illumination
    Olympus, Core Use Only
  • Laminar workflow station
    Thermo, Core Use Only
  • Multiple electrode array
    Axion BioSystems, Equipment Available For Use
  • NEON transfection system
    Thermofisher, Equipment Available For Use
  • Tissue incubator with high-O2 capacity
    Thermo, Core Use Only
  • Tissue incubator with low-O2 capacity
    Thermo, Core Use Only