Human Stem Cell and Gene Editing Core (HSCGE)
provides iPSC reprogramming, stem cell characterization and differentiation, and genome editing.
Contacts
Wei Niu, PhD
734-615-8215
weiniu@med.umich.edu
Location
BSRB (Rm 5371)
109 Zina Pitcher Pl, Ann Arbor, MI
Medical School
Who We Serve
University of Michigan Researchers and External Researchers
Core Summary
Founded in 2015, the HSCGE core is a well-established research core facility at University of Michigan Medical School. Our state-of-the-art facility is located on the 5th floor of BSRB. We provide a wide range of high-quality services (iPSC reprogramming, stem cell characterization, genome editing, brain organoids, and stem cell differentiation) and access to advanced instrumentation, methodologies, and expertise. Our personnel are very friendly and extremely well trained in stem cell biology as well as neuroscience applications. We serve investigators from UM, and researchers from external research centers as well as industry throughout the United States. We work with both the IRB and the HPSCRO committees and can provide regulatory advice to investigators.
Services
- 2D monolayer differentiation
cortical excitatory neurons, dopaminergic neurons, inhibitory neurons, motor neurons,
- 3D organoid differentiation
cortical organoid, thalamus organoid, ventral organoid,
- CRISPR/Cas9 genome/gene editing
knock in point mutations and epitope tagging (HDR), knockout (NHEJ),
- Facility usage
- Generate iNeurons
cortical excitatory neurons, inhibitory neurons,
- hESC/hiPSC expansion/freezing
- iPSC derivation from patients and controls
skin fibroblast, whole blood,
- iPSC lines
female, male,
- Neuronal network activity assays on neurons derived from human stem cells
- Training
hESC/hiPSC maintenance, hESC/hiPSC transfection,
Equipment
- Biosafety cabinet
Thermo, Core Use Only - Centrifuge Sorval ST8
Thermo, Equipment Available for Use - Countess FL
Thermo, Core Use Only - ECO CLEANBENCH
Thermo, Core Use Only - EVOS FL Auto live imaging system
Thermofisher, Equipment Available For Use - Heraeus CO2 tissue culture incubators
Thermo, Core Use Only - IncuCyte living-cell analysis system
Sartorius, Equipment Available For Use - Inverted CKX53 microscope with LED illumination
Olympus, Core Use Only - Laminar workflow station
Thermo, Core Use Only - Multiple electrode array
Axion BioSystems, Equipment Available For Use - NEON transfection system
Thermofisher, Equipment Available For Use - Tissue incubator with high-O2 capacity
Thermo, Core Use Only - Tissue incubator with low-O2 capacity
Thermo, Core Use Only