BSB Imaging and Cell Analysis Core

Laser scanning confocal microscopes, transmission electron microscope, and flow cytometry access and training.

Contacts

Gregg Sobocinski
(734) 615-2034
BSBimaging@umich.edu

Location

Basement of the Biological Sciences Building
College of LSA, University of Michigan
1105 North University Ave
Ann Arbor, Michigan 48109-1085

Affiliations
Literature, Science and the Arts

Who We Serve

University of Michigan Researchers

Core Summary

The BSB Imaging and Cell Analysis Core provides instrumentation, training, and some consultation regarding fluorescence microscopy, super-resolution fluorescence imaging, TEM and analytical flow cytometry. Equipment available for shared use includes a Leica SP8 inverted laser scanning confocal system with STED super-resolution technology, an upright Leica SP5 laser scanning confocal system, a Jeol 1400 plus transmission electron microscope configured for biological cell and tissue imaging, and an Attune flow cytometer with four lasers and 16 channels.

Service Categories: Imaging

Services

No items found

Additional Services

This core offers microscope usage training and some specimen preparation consultation as it pertains to using our microscope imaging systems. The core is not able to provide actual specimen preparation, handling, or imaging services at this time.

Equipment

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Additional Equipment

This Leica SP8 inverted-microscope, laser scanning confocal imaging system is well-appointed, including the following features: White Light Laser (470-670nm), 405 Diode laser, 3D STED super resolution imaging (592, 660, and 775 nm lasers), gating, spectral detection, 2PMT detectors, 2 HyD GaAsP avalance detectors, high speed Resonant Scanner (8,000Hz), Auto Drift Control, stage top 18 degree chiller (reduces drift), Tokai Hit sample incubator chamber, and stage inserts for slides, culture dishes, well slides, and culture plates. Objectives include 10x dry, 40x oil, 63x oil, 93x glycerin Corr (STED), and 100x oil (STED) lenses. The system runs LAS X software and Huygens deconvolution processing software.

Our Leica SP5 upright-microscope, laser scanning confocal imaging system includes 405 Diode, 458, 476, 488, 514nm Argon gas, 561nm, and 633nm lasers. Other features include spectral detection, 3PMT detectors, 2 HyD GaAsP avalance detectors, high speed Resonant Scanner (8,000Hz), and stage inserts for accommodating slides, culture dishes, and well slides. Objectives include 10x dry 20x dry, 40x dry Corr, 20x aqueous dipping, 40x aqueous dipping, 63x glycerin Corr, and 100x oil lenses. The system runs LAS AF software.

Our Jeol 1400 plus transmission electron microscope (TEM) is configured for biological sample imaging using an AMT XR80 camera. Accessories include high-tilt specimen holder, correlative imaging software, and electron tomography module and software.

The Attune NXT flow cytometer is appointed with 4 lasers (405, 488, 561, and 637nm), up to 16 detection channels (depending on your color overlap), and the capacity to run 384 or 96 well plates with the new Cytkick Max autosampler. Accommodates all well types, but round or conical well bases are preferred.