Bru-Seq Lab
provides nascent RNA sequencing using four techniques: Bru-seq, BruChase-seq, BruUV-seq, and BruDRB-seq.
Contacts
Mats Ljungman, PhD
(734) 232-5438
BruseqCenter@umich.edu
Location
NCRC Building 520 (Rms 1361 & 1410)
1600 Huron Pkwy, Ann Arbor, MI
University of Michigan Office of Research
Who We Serve
University of Michigan Researchers and External Researchers
Core Summary
The University of Michigan Bru-seq Lab is a core facility that provides nascent RNA sequencing using four techniques: Bru-seq, BruChase-seq, BruUV-seq, and BruDRB-seq. This service is all-inclusive, starting with cells and ending with basic data analysis. In addition, users will have access to our exclusive pipeline to perform further data analysis.
Our areas of expertise are RNA isolation, cDNA library preparation, and sequencing data analysis.
Services
- Bromouridine labeling of cells and cell collection
- Bru-seq (RNA transcription)
- BruChase-seq (RNA Stability)
- BruDRB-seq (RNA elongation rates)
- BruUV-seq (identification of enhancers and transcription start sites)
- cDNA Library Preparation
- Sample mapping and data analysis
- Sequencing (using the University of Michigan Advanced Genomics Core)
- Total and nascent RNA isolation