Single Molecule Analysis in Real-Time (SMART) Center
provides single molecule detection and manipulation tools to track and analyze biomolecules.
Contacts
Damon Hoff, PhD
734-763-5449
hoffj@umich.edu
Location
Chemistry Building (Rm 3080)
930 N University Ave, Ann Arbor, MI
Literature, Science and the Arts
Who We Serve
University of Michigan Researchers and External Researchers
Core Summary
The Single Molecule Analysis in Real-Time (SMART) Center is an NSF-seeded, shared-use facility providing university researchers with single molecule detection and manipulation tools to track and analyze biomolecules with unprecedented detail. The SMART Center provides access to state-of-the art instrumentation, including single molecule spectroscopy and imaging, laser tweezers, and atomic force microscopy; as well as experienced support in experimental planning and analysis. We would also be happy to give your group a short demonstration of our capabilities and discuss potential applications.
Services
- Fluorescence fluctuation spectroscopy
Fluorescence correlation spectroscopy, Photon counting histogram, Single molecule FRET,
- Force spectroscopy
Atomic force microscopy, Optical tweezers,
- Imaging
Atomic force microscopy, Fluorescence lifetime imaging, Multicolor TIRF or widefield fluorescence imaging and particle tracking, PALM/STORM), Single molecule localization microscopy (e.g. DNA-PAINT), Super-resolution imaging (PALM/STORM/DNA-PAINT), Time-resolved confocal,
- Training
Data analysis and workflow development, Image analysis, Microscope use and data acquisition, Quantitative image analysis,
Equipment
- MiniTweezers Optical Tweezers
Custom-made, Equipment Available For Use - Single Particle Nanomanipulator, Optical Tweezers and Fluorescence Microscope
Custom-made, Equipment Available For Use - Single Particle Tracker TIRF/HILO Fluorescence Microscope (plus cellTIRF and accessories) IX81
Olympus, Equipment Available For Use - Table-top Atomic Force Microscope TT-AFM
AFM Workshop, Equipment Available For Use - Time-Resolved Confocal Microscope Alba-5
ISS, Equipment Available For Use