Single Molecule Analysis in Real-Time (SMART) Center

provides single molecule detection and manipulation tools to track and analyze biomolecules.

Contacts

Damon Hoff, PhD
734-763-5449
hoffj@umich.edu

Location

Chemistry Building (Rm 3080)
930 N University Ave, Ann Arbor, MI

Affiliations
Literature, Science and the Arts

Who We Serve

University of Michigan Researchers and External Researchers

Core Summary

The Single Molecule Analysis in Real-Time (SMART) Center is an NSF-seeded, shared-use facility providing university researchers with single molecule detection and manipulation tools to track and analyze biomolecules with unprecedented detail.  The SMART Center provides access to state-of-the art instrumentation, including single molecule spectroscopy and imaging, laser tweezers, and atomic force microscopy; as well as experienced support in experimental planning and analysis.  We would also be happy to give your group a short demonstration of our capabilities and discuss potential applications.

Service Categories: Cell-Based Research; Data Tools and Analysis ; Imaging

Services

  • Fluorescence fluctuation spectroscopy
    Fluorescence correlation spectroscopy, Photon counting histogram, Single molecule FRET,

  • Force spectroscopy
    Atomic force microscopy, Optical tweezers,

  • Imaging
    Atomic force microscopy, Fluorescence lifetime imaging, Multicolor TIRF or widefield fluorescence imaging and particle tracking, PALM/STORM), Single molecule localization microscopy (e.g. DNA-PAINT), Super-resolution imaging (PALM/STORM/DNA-PAINT), Time-resolved confocal,

  • Training
    Data analysis and workflow development, Image analysis, Microscope use and data acquisition, Quantitative image analysis,


Equipment

  • MiniTweezers Optical Tweezers
    Custom-made, Equipment Available For Use
  • Single Particle Nanomanipulator, Optical Tweezers and Fluorescence Microscope
    Custom-made, Equipment Available For Use
  • Single Particle Tracker TIRF/HILO Fluorescence Microscope (plus cellTIRF and accessories) IX81
    Olympus, Equipment Available For Use
  • Table-top Atomic Force Microscope TT-AFM
    AFM Workshop, Equipment Available For Use
  • Time-Resolved Confocal Microscope Alba-5
    ISS, Equipment Available For Use