FCVC Cell Regeneration Core Laboratory
The Frankel Cardiovascular Center Cell Regeneration Core's provides top-tier resources, expertise, and innovative services like New Approach Methodologies (NAMs). NAMs services offer advanced cell characterization, functional assays, and molecular analysis. The FCVC Cell Regeneration Core fosters collaboration and excellence across the Medical Schools and other Colleges across the University of Michigan.
RRID
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University of Michigan FCVC Cell Regeneration Core Laboratory RRID:SCR_026728
Serving
External Researchers, U-M Researchers
Service Categories
Cell-Based Research, Data Tools and Analysis, Drug Discovery & Pharmacology, Education and Training, Imaging, Microscopy, and Analysis, Molecular Biology, Phenotyping, Physiology, Stem Cell
Services
- 3D Micro-tissue Generation (1 Plate)
- 3D Micro-tissue optical mapping (1 Plate)
- cDNA and Q-PCR
- Cryobank Storage Fee - Monthly
- Cytation data retrieval
- Cytation monitoring set up
- Data Analysis/Consultation
- DNA extraction and quantitation
- High fidelity PCR
- hiPSC - Pancreatic Differentiation 2 plates( 6 well) ~ 7000 islets
- hiPSC cryopreservation
- hiPSC culture/expansion
- hiPSC derivation from adherent cells (fibrobalast and others)
- hiPSC derivation from PBMCs
- hiPSC passage
- hiPSC thaw
- CRISPR correction of hiPSC lines
- hiPSC-CM cryopreservation from CDML3
- hiPSC-CM cryopreservation from MACS
- hiPSC-CM cytotoxicity imaging (time lapse)
- hiPSC-CM differentiation atrial
- hiPSC-CM differentiation ventricular
- hiPSC-CM Electrophysiology assay (96 well format)
- hiPSC-CM MACS
- hiPSC-CM purification Metabolic/CDML3
- hiPSC-CM replating
- hiPSC-purkinje
- Imaging, Real-Time
- Incucyte analysis set up and data retrieval
- Incucyte Monitoring
- Karyotyping
- Optical Mapping
- Optical Mapping drug discovery
- Optical Mapping drug discovery with restitution curve
- Optical Mapping restitution curve
- PBMC isolation and cryopreservation
- Protein extraction (5 to 10 samples)
- RNA extraction and quantitation
- Skin fibroblast expansion
- Staining of Sections
- Training and Technical Support
- Transfer of Cells to PI
- Western Blots (comassie, equal loadin control and 1 target)
- Western Blots analysis
Equipment
- AutoMACS
- Cytation 5
- Biospa 8
- Incucyte
- Mapping lab Optical Mapping System