Bru-Seq Lab

provides nascent RNA sequencing using four techniques: Bru-seq, BruChase-seq, BruUV-seq, and BruDRB-seq.

Contacts

Mats Ljungman, PhD
(734) 232-5438
BruseqCenter@umich.edu

Location

NCRC Building 520 (Rms 1361 & 1410)
1600 Huron Pkwy, Ann Arbor, MI

Affiliations
University of Michigan Office of Research

Who We Serve

University of Michigan Researchers and External Researchers

Core Summary

The University of Michigan Bru-seq Lab is a core facility that provides nascent RNA sequencing using four techniques: Bru-seq, BruChase-seq, BruUV-seq, and BruDRB-seq. This service is all-inclusive, starting with cells and ending with basic data analysis. In addition, users will have access to our exclusive pipeline to perform further data analysis.

Our areas of expertise are RNA isolation, cDNA library preparation, and sequencing data analysis.

Service Categories: Cell-Based Research Service Categories: Gene Sequencing, Expression and Analysis

Services

Bromouridine labeling of cells and cell collection

Bru-seq (RNA transcription)

BruChase-seq (RNA Stability)

BruDRB-seq (RNA elongation rates)

BruUV-seq (identification of enhancers and transcription start sites)

cDNA Library Preparation

Sample mapping and data analysis

Sequencing (using the University of Michigan Advanced Genomics Core)

Total and nascent RNA isolation


Equipment

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