Pluripotent Stem Cell Core
trains scientists in the culture of human pluripotent stem cells (hPSC).
Contacts
Cindy DeLong, PhD
734-763-5557
cjweaver@umich.edu
Location
BSRB (Rms 3661, 3671, 3681, 3880, 3888)
109 Zina Pitcher Pl, Ann Arbor, MI
Medical School
Who We Serve
University of Michigan Researchers and External Researchers
Core Summary
The Core lab focuses on training scientists in the culture of human pluripotent stem cells (hPSC). Students spend one intensive week training in the lab with the help of stem cell specialists. This one week course includes mostly hands-on training to learn how to culture hPSCs on Matrigel. Students will learn how to identify pluripotent vs differentiated colonies and what procedures to use to maintain pluripotent cultures. Students will also practice thawing and freezing cultures, and as time and interest allows, practice common differentiation culture techniques such as making EBs or single cell monolayers. The training manual also covers pluripotency characterization and routine quality control.
Requirements: At least one year of experience with mammalian cell culture.
Cost: $1260 for one week of training with hPSC on Matrigel. Additional time can be added on a weekly or monthly basis at $900/mo (as space permits) until the student is comfortable to transfer their skills back into their own lab.
(If training with the use of mouse embryonic fibroblast (MEF) feeder cells is needed, contact cjweaver@umich.edu).
Services
- Training
Maintaining stem cells in a pluripotent state, Passaging and characterizing pluripotent cells, Practicing common differentiation culture techniques such as making EBs or single cell monolayers,
Equipment
- Camera on stereomicroscope DS-2Mv
Nikon, Core Use Only - Inverted fluorescent microscope DMi8
Leica, Core Use Only - Inverted microscope DMi1
Leica, Core Use Only - Stereomicroscope MZ95
Leica, Core Use Only