Pluripotent Stem Cell Core

trains scientists in the culture of human pluripotent stem cells (hPSC).

Contacts

Cindy DeLong, PhD
734-763-5557
cjweaver@umich.edu

Location

BSRB (Rms 3661, 3671, 3681, 3880, 3888)
109 Zina Pitcher Pl, Ann Arbor, MI

Affiliations
Medical School

Who We Serve

University of Michigan Researchers and External Researchers

Core Summary

The Core lab focuses on training scientists in the culture of human pluripotent stem cells (hPSC). Students spend one intensive week training in the lab with the help of stem cell specialists. This one week course includes mostly hands-on training to learn how to culture hPSCs on Matrigel. Students will learn how to identify pluripotent vs differentiated colonies and what procedures to use to maintain pluripotent cultures. Students will also practice thawing and freezing cultures, and as time and interest allows, practice common differentiation culture techniques such as making EBs or single cell monolayers. The training manual also covers pluripotency characterization and routine quality control.

Requirements: At least one year of experience with mammalian cell culture.

Cost: $1260 for one week of training with hPSC on Matrigel. Additional time can be added on a weekly or monthly basis at $900/mo (as space permits) until the student is comfortable to transfer their skills back into their own lab.

(If training with the use of mouse embryonic fibroblast (MEF) feeder cells is needed, contact cjweaver@umich.edu).

Service Categories: Cell-Based Research

Services

  • Training
    Maintaining stem cells in a pluripotent state, Passaging and characterizing pluripotent cells, Practicing common differentiation culture techniques such as making EBs or single cell monolayers,


Equipment

  • Camera on stereomicroscope DS-2Mv
    Nikon, Core Use Only
  • Inverted fluorescent microscope DMi8
    Leica, Core Use Only
  • Inverted microscope DMi1
    Leica, Core Use Only
  • Stereomicroscope MZ95
    Leica, Core Use Only