BRCF Proteomics & Peptide Synthesis Core

offers research services in qualitative and quantitative proteomics including pathway analysis.

Contacts

Henriette A. Remmer, PhD
734-763-6285
hremmer@umich.edu

Location

MSRB II (Rms C568 & C570C)
1150 W Medical Center Dr, Ann Arbor, MI

Affiliations
Medical School

Who We Serve

University of Michigan Researchers and External Researchers

Core Summary

The Proteomics & Peptide Synthesis Core is a full service Core and provides proteomics support from scientific consultation/experimental design and sample preparation all the way to data analysis and interpretation. We provide the entire range of qualitative and quantitative proteomics services. We accept a wide range of sample formats including complex biological and clinical research samples in unprocessed form (e.g. broad variety of tissues, cells, cell supernatants and conditioned media, blood serum,  urine) as well as routine samples ( e.g. SDS-PAGE gel bands, eluates from affinity chromatography or IP, beads). Pathway Analysis is also available.

We also provide N-terminal (Edman) sequencing services and Amino Acid Analysis of proteins and peptides.

Our custom peptide synthesis services range from routine peptides to specialized peptides such as long, hydrophobic, cyclic, or stapled peptides. We provide quotations within one business day at very competitive prices. Please email hremmer@umich.edu with your peptide sequence, desired peptide yield, and purity.

Service Categories: Chemical, Material and Protein Characterization; Data Tools and Analysis

Services

  • Amino acid analysis

  • Mass spectrometry
    LC-MS, LC-MS/MS, MALDI-TOF, Protein mass spectrometry,

  • Pathway Analysis

  • Peptide synthesis

  • Post-translational modifications mapping

  • Protein identification

  • Protein quantitation
    Label-free quantitation, TMT quantitation,

  • Protein sequencing
    Edman sequencing, Electrospray, MALDI-TOF/TOF,


Equipment

  • nanoscale liquid chromatography system UPLC
    Waters, Core Use Only
  • Orbitrap Exploris 480 mass spectrometer
    Thermo Scientific, Core Use Only
  • Orbitrap Fusion Lumos Tribrid mass spectrometer
    Thermo Scientific, Core Use Only
  • Orbitrap Q-Exactive mass spectrometer
    Thermo Scientific, Core Use Only